Selection of coffee genotypes resistant to rooster's eye [Mycena citricolor (Berk. & M.A. Curtis) Sacc.]
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Abstract
To evaluate the use of oxalic acid in selecting genotypes resistant to coffee leaf rust (Mycena citricolor (Berk. & M.A.Curtis) Sacc.), an experiment was conducted using a completely randomized design (CRD) with an unbalanced layout and 55 treatments at the UNESUM Biotechnology Laboratory. Ten genotypes were placed per tray, with two individual leaflets per genotype, for a total of 11 trays. Each leaflet was inoculated with three drops on each side of the midrib at a concentration of 2.25 g/150 mL of oxalic acid. Lesion size (LS) was measured in millimeters using a Vernier caliper starting on the third day, with daily readings continuing for six days. Using the LS data, the area under the relative lesion progression curve (AULRP) was determined as a relative measure over time. Analysis of variance (ANOVA) and comparison of means were performed using Tukey’s multiple range test (P < 0.05), after verifying that the assumptions of normality and homogeneity of variances were met. Additionally, the best-fit curve was determined using the regression coefficient (R²). The results showed that genotypes 021-101-4 and 021-101-3 exhibited high resistance to necrosis caused by oxalic acid. Genotype 021-106-4 was the most susceptible, as were the cultivars Gheisha and Típica; thus, different levels of resistance to oxalic acid were identified, ranging from the most resistant to the most susceptible. Both the progenies and the parent lines evaluated had a logarithmic regression fit.
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