Prevalence of Bovine Tuberculosis (BTB) by post-mortem inspection and bacteriological culture in the municipal slaughterhouse of Mejía canton (Pichincha-Ecuador)

Main Article Content

Diego Martín Cushicóndor-Collaguazo
Roberto Darwin Coello-Peralta
Estefania Ortega
Enrique Xavier Rodriguez Burnham

Abstract

This cross-sectional study was carried out at the Municipal Slaughterhouse of Canton Mejía between May 27 - July 17, 2013 in order to estimate the apparent prevalence of TBB tuberculosis in slaughtered cattle based on macroscopic post-mortem inspection and isolation of M. bovis. In total, 395 heads of cattle were examined during the study period, of which 4 animals were diagnosed with tuberculous lesions compatible with the disease at the lung level, giving an apparent prevalence of 1.01% (IC95%: 0.28% - 2.57 %); while, through bacteriological culture, isolates compatible with M. bovis were identified in 6 animals, giving an apparent prevalence of 1.52% (IC95% = 0.56% – 3.28%). Risk factors such as age, biotype, sex and origin of the animals were not significant in the apparent prevalence of the disease. In conclusion, a significant prevalence of macroscopic lesions and of M. bovis was obtained without obtaining significant association of the factors that predispose to a greater presentation of visible lesions and of the pathogen, therefore, control strategies could not be redirected in the different populations that could be at risk.

Downloads

Download data is not yet available.

Article Details

How to Cite
Cushicóndor-Collaguazo, D. M., Coello-Peralta, R. D., Ortega, E., & Rodriguez Burnham, E. X. (2023). Prevalence of Bovine Tuberculosis (BTB) by post-mortem inspection and bacteriological culture in the municipal slaughterhouse of Mejía canton (Pichincha-Ecuador). Centrosur Agraria, 1(16). https://doi.org/10.37959/revista.v1i16.233
Section
Articles

References

Acha, P. N., & Szyfres, B. (2001). Zoonoses and communicable diseases common to man and animals (3rd ed., vol. 1., pp. 266-283). Washington D.C.: Pan American Health Organization (PAHO).

Aliyu, M. M., Adamu, J. Y., & Bilyaminu, Y. A. (2009). Current prevalence of tuberculous lesions among slaughtered cattle in Northeastern States of Nigeria. Revue d'Élevage et de Médecine Vétérinaire Des Pays Tropicaux, 62(1), 13-16.

Amanfu, W. (2006). The situation of tuberculosis and tuberculosis control in animals of economic interest. Tuberculosis Edinburgh Scotland, 86(3-4), 330-335.

Ameni, G., Aseffa, A., Engers, H., Young, D., Gordon, S., Hewinson, G., & Vordermeier, M. (2007). High prevalence and increased severity of pathology of bovine tuberculosis in Holsteins compared to zebu breeds under field cattle husbandry in central Ethiopia. Clinical and Vaccine Immunology CVI, 14(10), 1356-1361.

Araújo, C. P., Fugimura, C. Q., de Prince, K. A., Gonçalves, K., & Alves, A. L. (2005). Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil. Memorias Do Instituto Oswaldo Cruz, 100(7), 749-752.

Baptista, F., Moreira, E. C., Santos, W. L. M., & Naveda, L. A. B. (2004). Prevalence of tuberculosis in cattle slaughtered in Minas Gerais. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 56(5), 577-580.

Biet, F. B., Boschiroli, M. L. B., Thorel, M. F., & Guilloteau, L. A. G. (2005). Review article Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC). Veterinary Research, 36, 411-436.

Biffa, D., Inangolet, F., Oloya, J., Asseged, B., Badaso, M., Yilkal, A., & Skjerve, E. (2009). Prevalence of bovine tuberculosis in Ethiopian slaughter cattle based on post-mortem examination. Tropical Animal Health and Production, 41(5), 755-65.

Cadmus, S. I. B., Agada, C. A., Onoja, I. I., & Salisu, I. (2010). Risk factors associated with bovine tuberculosis in some selected herds in Nigeria. Tropical Animal Health and Production, 42(4), 547-9.

Cleaveland, S., Shaw, D. J., Mfinanga, S. G., Shirima, G., Kazwala, R. R., Eblate, E., & Sharp, M. (2007). Mycobacterium bovis in rural Tanzania: Risk factors for infection in human and cattle populations. Tuberculosis, 87(1), 30-43.

Corner, L. A. L., Gormley, E., & Pfeiffer, D. U. (2012). Primary isolation of Mycobacterium bovis from bovine tissues: conditions for maximising the number of positive cultures. Veterinary Microbiology, 156(1-2), 162-71.

de Kantor, I. N., & Ritacco, V. (2006). An update on bovine tuberculosis programmes in Latin American and Caribbean countries. Veterinary Microbiology, 112(2-4), 111-118.

de Kantor, I. N., Torres, P. M., Morcillo, N., Imaz, M. S., & Sequeira, M. D. (2012). Zoonotic tuberculosis in Argentina. Medicine. Retrieved from http://www.scielo.org.ar/pdf/medba/v72n6/v72n6a14.pdf

Echeverría, G., Ron, L., León, A. M., Espinosa, W., Benítez-Ortiz, W., Proaño-Pérez, F. (2014). Prevalence of bovine tuberculosis in slaughtered cattle identified by nested-PCR in abattoirs from two dairy areas of Ecuador. Tropical animal health and production.

Food and Agriculture Organization of the United Nations (FAO). (2012). Bovine tuberculosis at the animal-human-ecosystem interface. Transboundary Animal Diseases Bulletin. Available in www.fao.org/empres

Gathogo, S. M., Kuria, J. K., & Ombui, J. N. (2012). Prevalence of bovine tuberculosis in slaughter cattle in Kenya: a postmortem, microbiological and DNA molecular study. Tropical Animal Health and Production, 44(7), 1739-1744.

Hernández, D. A. J., Rentería, E. T., López, V. G., & Montaño, H. M. (1997). An abattoir monitoring system for diagnosis of tuberculosis in cattle in Baja California, Mexico. Journal of the American Veterinary Medical Association, 211(6), 709-711.

Humblet, M.-F., Boschiroli, M. L., & Saegerman, C. (2009). Classification of worldwide bovine tuberculosis risk factors in cattle: a stratified approach. Veterinary Research, 40(5), 24.

Inangolet, F. O., Demelash, B., Oloya, J., Opuda-Asibo, J., & Skjerve, E. (2008). A cross-sectional study of bovine tuberculosis in the transhumant and agro-pastoral cattle herds in the border areas of Katakwi and Moroto districts, Uganda. Tropical Animal Health and Production, 40(7), 501-508.

Kazwala, R. R. R., Kambarage, D. M., Daborn, C. J., Nyange, J., Jiwa, S. F., & Sharp, J. M. (2001). Risk factors associated with the occurrence of bovine tuberculosis in cattle in the Southern Highlands of Tanzania. Veterinary Research Communications, 25(8), 609-614.

Koro, F. K., Foko, E., Ngatchou, A. F., Eyangoh, S., & Etoa, F. (2013). First Insight into the Current Prevalence of Bovine Tuberculosis in Cattle Slaughtered in Cameroon: The Case of Main Abattoirs of Yaoundé and Douala. British Microbiology Research Journal, 3(3), 272-279.

Latini, O., Canal, A. M., Ferrara, M. E., Sequeira, M. D., Sequeira, G., Bagnaroli, R., & Torres, P. (1997). Reliability in the determination of prevalence of Mycobacterium bovis infection in cattle by seizures in slaughterhouses. Archives of Veterinary Medicine, 29(2), 197-204.

Lévy-Frébault, V. V, & Portaels, F. (1992). Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. International Journal of Systematic Bacteriology, 42(2), 315-323.

Mellau, L. S. B., Nonga, H. E., & Karimuribo, E. D. (2010). A slaughterhouse survey of lung lesions in slaughtered stocks at Arusha, Tanzania. Preventive Veterinary Medicine, 97(2), 77-82.

Menin, Á., Fleith, R., Reck, C., Marlow, M., Fernandes, P., Pilati, C., & Báfica, A. (2013). Asymptomatic cattle naturally infected with Mycobacterium bovis present exacerbated tissue pathology and bacterial dissemination. PloS One, 8(1), e53884.

Michel, A. L., Müller, B., & Van Helden, P. D. (2010). Mycobacterium bovis at the animal-human interface: a problem, or not? Veterinary Microbiology, 140(3-4), 371-81.

Milian-Suazo, F., Salman, M. D., Ramirez, C., Payeur, J. B., Rhyan, J. C., & Santillan, M. (2000). Identification of tuberculosis in cattle slaughtered in Mexico. American Journal of Veterinary Research, 61(1), 86-89.

Miller, J. M., Jenny, A. L., & Payeur, J. B. (2002). Polymerase chain reaction detection of Mycobacterium tuberculosis complex and Mycobacterium avium organisms in formalin-fixed tissues from culture-negative ruminants. Veterinary Microbiology, 87(1), 15-23.

Nava, O., & Prieto, L. (2001). Bacteriological diagnosis of pulmonary tuberculosis (review). Kasmera, 1-15.

Office International des Epizooties (OIE). (2021). Bovine tuberculosis. OIE Terrestrial Manual. Retrieved from http://www.oie.int/fileadmin/Home/esp/Health_standards/tahm/2.04.07_BOVINE_TB.pdf

Oloya, J., Kazwala, R., Lund, A., Opuda-Asibo, J., Demelash, B., Skjerve, E., ... Djønne, B. (2007). Characterisation of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda. BioMed Central Microbiology, 7(95), 1-7.

Omer, M. K., Skjerve, E., Woldehiwet, Z., & Holstad, G. (2001). A cross-sectional study of bovine tuberculosis in dairy farms in Asmara, Eritrea. Tropical Animal Health and Production, 33(4), 295-303.

Pace, J. E., & Wakeman, D. L. (2003). Determining the age of cattle by their teeth. Journal of the American Veterinary Medical Association, 121(909), 483.

Palmer, M. V, & Ray Waters, W. (2006). Advances in bovine tuberculosis diagnosis and pathogenesis: what policy makers need to know. Veterinary Microbiology, 112(2-4), 181-90.

Pourrain A. (2007). Biotypes in Cattle. ArgenINTA Foundation. Argentine site of animal production. Retrieved June 4, 2013. Retrieved from http://www.produccion-animal.com.ar/informacion_tecnica/exterior/20-biotipos.pdf

Proaño-Pérez, F., Rigouts, L., Brandt, J., Dorny, P., Ron, J., Chavez, M.-A., ... Benitez-Ortiz, W. (2006). Preliminary observations on Mycobacterium spp. in dairy cattle in Ecuador. The American Journal of Tropical Medicine and Hygiene, 75(2), 318-23.

Proaño-Pérez, F., Benitez-Ortiz, W., Desmecht, D., Coral, M., Ortiz, J., Ron, L., ... Linden, A. (2011). Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador. Preventive Veterinary Medicine, 101(1-2), 65-72.

Regassa, A., Tassew, A., Amenu, K., Megersa, B., Abunna, F., Mekibib, B., ... & Ameni, G. (2010). A cross-sectional study on bovine tuberculosis in Hawassa town and its surroundings, Southern Ethiopia. Tropical animal health and production, 42(5), 915-920.

Shitaye, J. E., Getahun, B., Alemayehu, T., Skoric, M., & Treml, F. (2006). A prevalence study of bovine tuberculosis by using abattoir meat inspection and tuberculin skin testing data, histopathological and IS 6110 PCR examination of tissues with tuberculous lesions in cattle in Ethiopia. Veterinarni Medicina-PRAHA, 51(11), 512-522.

Toledo P., Milian F., Santillan M. Ramirez I. (1999). Isolation and identification of Mycobacterium bovis from expectoration samples of human patients with chronic respiratory problems. Vet. Mex. 30(3) 227.

Torres, P. M. (2009). Epidemiological surveillance system through the detection of bovine tuberculosis at slaughter for the epidemiological characterization and control of the disease in the Province of Entre Ríos. Retrieved from http://www.senasa.gov.ar/Archivos/File/File3383-tuber-bovina-tesis.pdf

Most read articles by the same author(s)