Response of seed imbibition time of three citrus rootstocks with different concentrations of Pectimorf® during germination
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Abstract
Finding safer germination methods is essential in citrus fruits, so the aim of this research was to evaluate the response of the imbibition time of seeds from three citrus rootstocks with different concentrations of Pectimorf® on germination. An experiment was conducted in the laboratory with two study factors: Factor A: rootstock seed (A1: Cleopatra mandarin, A2: rough lemon, A3: mandarin lime) and Factor B: pectic oligosaccharide concentration (B1: 0 mgL-1, B2: 5 mgL-1, B3: 10 mgL-1, B4: 20 mgL-1). The treatments were distributed in a completely randomised experimental design (CRD) in a 3x4 factorial arrangement, with 12 treatments. The percentage of germination (PDG), radicle wet weight (PHDR), radicle dry weight (PSDR), vigour index (IDV) and seed growth dynamics were evaluated. The analyses were performed in Infostat and Excel. The results showed that the lime mandarin rootstock performed best at a dose of 20.00 mgL-1. The rough lemon performed best at doses of 5.00, 10.00 and 20.00 mgL-1, and the Cleopatra mandarin at doses of 10.00 and 20.00 mgL-1. With regard to germination speed, rough lemon responded best to 5.00 mgL-1 of polysaccharides, followed by rough lemon with 10.00 mgL-1 of oligosaccharides. The application of the biostimulant Pectimorf® to lime mandarin seeds had the best pHDR weight- .
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