Diagnosis of tuberculosis in non-human primates through intradermal testing in wildlife centers in the city of Guayaquil, Ecuador
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Abstract
Tuberculosis is a zoonotic disease of public health danger. Non-human primates are a susceptible group, being the objective of the present investigation to determine positive cases of tuberculosis and to correlate the risk factors with the degrees of reaction presented. Intradermal tuberculin was the test of choice in the present study, oriented under an experimental intention, quantitative approach, applied type. The total population of non-human primates in captivity in two Wild Animal Rescue Centers in the city of Guayaquil was examined by inoculating 13 specimens with 0.1 ml of intradermal Bovine PPD Tuberculin in the right eyelid. Subsequently, the degrees of postinoculation reaction were evaluated under a criterion of 0 to 5 during 24, 48 and 72 hours. The result was 0% of positive cases detected, with grade 1 reaction in two primates, attributing the reaction according to the clinical history, to the close human-animal contact as a possible risk factor. Thus concluding that there is no difference between the risk factors with respect to the reactions presented by the primates, however, there is a difference in the number of reactions in relation to the registered primates.
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References
Albornoz Villacrés, V. P. (2012). Determinación de la prevalencia de mycobacterium spp. Mediante la aplicación de la prueba de tuberculinización, y análisis de factores de riesgo en primates y felinos mantenidos en cautiverio en el zoológico de Guayllabamba [Tesis de pregrado]. Universidad de las Américas. https://dspace.udla.edu.ec/handle/33000/2848
Aranaz Martín, Alicia., Domínguez Rodríguez, L., Mateos García, A., Suárez Fernández, G., & animal), U. C. de M. F. de V. D. P. A. I. (Sanidad. (2002). Aplicación de la técnica de PCR (Reacción en cadena de la polimerasa) en el diagnóstico y epidemiología de la tuberculosis en animales. Universidad Complutense de Madrid, Servicio de Publicaciones. http://hdl.handle.net/20.500.14352/63036
Azé, J., Sola, C., Zhang, J., Lafosse-Marin, F., Yasmin, M., Siddiqui, R., Kremer, K., van Soolingen, D., & Refrégier, G. (2015). Genomics and Machine Learning for Taxonomy Consensus: The Mycobacterium tuberculosis Complex Paradigm. PLOS ONE, 10(7), e0130912. https://doi.org/10.1371/journal.pone.0130912
Bonovska, M., Tzvetkov, Y., Najdenski, H., & Bachvarova, Y. (2005). PCR for Detection of Mycobacterium tuberculosis in Experimentally Infected Dogs. Journal of Veterinary Medicine, Series B, 52(4), 165–170. https://doi.org/10.1111/j.1439-0450.2005.00839.x
Bucsan, A. N., Mehra, S., Khader, S. A., & Kaushal, D. (2019). The current state of animal models and genomic approaches towards identifying and validating molecular determinants of Mycobacterium tuberculosis infection and tuberculosis disease. Pathogens and Disease, 77(4). https://doi.org/10.1093/femspd/ftz037
Capuano, S. V., Croix, D. A., Pawar, S., Zinovik, A., Myers, A., Lin, P. L., Bissel, S., Fuhrman, C., Klein, E., & Flynn, J. L. (2003). Experimental Mycobacterium tuberculosis Infection of Cynomolgus Macaques Closely Resembles the Various Manifestations of Human M. tuberculosis Infection. Infection and Immunity, 71(10), 5831–5844. https://doi.org/10.1128/IAI.71.10.5831-5844.2003
Cardona, P. J. (2018). Pathogenesis of tuberculosis and other mycobacteriosis. Enfermedades Infecciosas y Microbiologia Clinica, 36(1), 38–46. https://doi.org/10.1016/j.eimc.2017.10.015
Cely, G. E. E., Valencia-Aguirre, S., & Lugo, W. O. V. (2011). Prevalencia de tuberculosis en primates en cautiverio. CES Medicina Veterinaria y Zootecnia, 6(2), 62–72. https://revistas.ces.edu.co/index.php/mvz/article/view/2057
Churchyard, G., Kim, P., Shah, N. S., Rustomjee, R., Gandhi, N., Mathema, B., Dowdy, D., Kasmar, A., & Cardenas, V. (2017). What We Know About Tuberculosis Transmission: An Overview. The Journal of Infectious Diseases, 216(suppl_6), S629–S635. https://doi.org/10.1093/infdis/jix362
De la Torre, S. (2010). Los primates ecuatorianos, estudios y perspectivas. ACI Avances En Ciencias e Ingenierías, 2(2). https://doi.org/10.18272/aci.v2i2.30
De Waard, J. H. (2005). Tuberculosis bovina. Manual de Ganaderia Doble Propósito. Gonzalez-Stagnaro E. Soto-Belloso (Eds.). Maracaibo Venezuela: Ediciones Astro Data SA.
Foreman, T. W., Mehra, S., Lackner, A. A., & Kaushal, D. (2018). Translational Research in the Nonhuman Primate Model of Tuberculosis. ILAR Journal, 58(2), 151–159. https://doi.org/10.1093/ilar/ilx015
Gong, W. P., Yang, Y. R., Luo, Y., Li, N., Bai, X. J., Liu, Y. P., Zhang, J. X., Chen, M., Zhang, C. L., & Wu, X. Q. (2017). Alerta por infección por Mycobacterium tuberculosis de macacos rhesus en un zoológico salvaje de China. Experimental Animals , 66(4), 357–365. https://doi: 10.1538/expanim.16-0095.
Howie, S. R. C., Ebruke, B. E., McLellan, J. L., Deloria Knoll, M., Dione, M. M., Feikin, D. R., Haddix, M., Hammitt, L. L., Machuka, E. M., Murdoch, D. R., O’Brien, K. L., Ofordile, O., Olutunde, O. E., Parker, D., Prosperi, C., Salaudeen, R. A., Shamsul, A., Mackenzie, G., Antonio, M., & Zaman, S. M. A. (2021). The Etiology of Childhood Pneumonia in The Gambia. Pediatric Infectious Disease Journal, 40(9S), S7–S17. https://doi.org/10.1097/INF.0000000000002766
Ministerio del Ambiente. (2019). Protocolo para el censo y monitoreo de primates y guacamayo verde de la Costa ecuatoriana https://www.ambiente.gob.ec/wp-content/uploads/downloads/2019/02/protocolo-Primates-y-guacamayos-09-08-18.pdf-
Ministerio de Salud Pública. (2018). Boletín Anual Tuberculosis. https://www.salud.gob.ec/wp-content/uploads/2019/03/informe_anual_TB_2018UV.pdf
Nagar, R., Pande, S., & Khopkar, U. (2006). Intradermal tests in dermatology-I: Tests for infectious diseases. Indian Journal of Dermatology, Venereology and Leprology, 72(6), 461. https://doi.org/10.4103/0378-6323.29351
Namasivayam, S., Kauffman, K. D., McCulloch, J. A., Yuan, W., Thovarai, V., Mittereder, L. R., Trinchieri, G., Barber, D. L., & Sher, A. (2019). Correlation between Disease Severity and the Intestinal Microbiome in Mycobacterium tuberculosis-Infected Rhesus Macaques. MBio, 10(3). https://doi.org/10.1128/mBio.01018-19
Obaldía N, Nuñez M, Montilla S, Otero W, Marin C. (2018). Tuberculosis (TB) outbreak in a closed Aotus monkey breeding colony: Epidemiology, diagnosis and TB screening using antibody and interferon-gamma release testing, Comparative Immunology, Microbiology and Infectious Diseases https://doi.org/10.1016/j.cimid.2018.06.007
OMS. (2023, November 23). Tuberculosis. Organización Mundial de La Salud. https://www.who.int/es/news-room/fact-sheets/detail/tuberculosis
Proaño-Pérez, F., Rigouts, L., Brandt, J., Dorny, P., Ron, J., Chavez, M.-A., Rodriguez, R., Fissette, K., Van Aerde, A., & Portaels, F. (2006). Preliminary observations on Mycobacterium spp. in dairy cattle in Ecuador. The American Journal of Tropical Medicine and Hygiene, 75(2), 318–323. https://doi.org/10.4269/ajtmh.2006.75.318
Trivedi, A., Singh, N., Bhat, S. A., Gupta, P., & Kumar, A. (2012). Chapter 4 - Redox
Biology of Tuberculosis Pathogenesis. In R. K. Poole (Ed.), Advances in Microbial Physiology (Vol. 60, pp. 263–324). Academic Press. https://doi.org/https://doi.org/10.1016/B978-0-12-398264-3.00004-8
World Organisation for Animal Health (WOAH). (2022). Manual Terrestre de la OIE 2022. Tuberculosis de los Mamíferos (Infección por el Complejo Mycobacterium Tuberculosis) Capítulo 3 .1. 1 3. Obtenido de https://www.woah.org/fileadmin/Home/esp/Health_standards/tahm/3.01.13_Mammalian_tuberculosis.pdf