Physicochemical characterization of propolis collected by Apis mellifera L (Hymenoptera: Apidae) in Pacho and Bogotá Cundinamarca
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Abstract
The objective of this work was to characterize and implement a reliable, viable and effective physicochemical methodology for the quality of Apis mellifera propolis collected in two apiaries located in Pacho and Suba-Bogotá, Cundinamarca, Colombia. A qualitative analysis was designed and validated to diagnose and evaluate the quality of propolis for laboratory analysis. Samples were collected by the scraping method, ethanolic extracts of propolis (EEP) were obtained and results were compared under several Latin American standards (Cuban, Brazilian and Salvadoran standards). The presence of phenols and flavonoids was determined by means of solubility in lead acetate, ferric chloride, sodium hydroxide, pW, humidity, oxidizing property, UV-vis spectroscopy profiles, pH, mechanical masses, dry mass, percentage of phenolic compounds, ashes, determination of major and minor minerals and quantity of flavonoids in quercetin. The results showed that the solubility in lead acetate and sodium hydroxide were positive for the 10 samples in duplicate, plus the 3 validation samples; the results showed significant differences between locations and compared with current standards.
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References
Bastos, E. M. M. A. F., Guzmán, D., Figueroa, J., Tello, J., & Scoaris, D. de O. (2011).Antimicrobial and physicochemical characterization of propolis from Apis mellifera L. (Hymenoptera: Apidae) from the Colombian Andean region. Acta Biologica Colombiana, 16(1), 175-184.
Betances, E. V. (2018). Characterization of the chemical composition and functional properties of propolis. Retrieved from https://gredos.usal.es/bitstream/handle/10366/139784/REDUCIDA_Prop%c3%b3leos.pdf?sequence=1&isAllowed=y
Carrillo, M. L., Castillo, L. N., & Mauricio, R. (2011). Evaluation of the Antimicrobial Activity of Propolis Extracts from the Huasteca Potosina (Mexico). Informacion Tecnologica, 22(5), 21-28. https://doi.org/10.4067/S0718-07642011000500004. https://doi.org/10.4067/S0718-07642011000500004.
Delgado Aceves, M. D. L., Andrade Ortega, J. Á., & Ramírez Barragán, C. A. (2018). Physicochemical characterization of propolis collected in Bosque La Primavera Zapopan, Jalisco. Revista Mexicana de Ciencias Forestales, http://cienciasforestales.inifap.gob.mx/editorial/index.php/forestales/article/view/270/289.
Geometry, Riemannian, G. (2008). Russian branch norm. Norma agricola de Rusai, 49, 5.
Gil, J., Viloria, J., Durango, D., & Garcia, C. (2012). Physicochemical characterization of propolis from the Bajo Cauca Antioqueño region (Antioquia, Colombia). Biotechnology in the Agricultural and Agroindustrial Sector: BSAA, 10(1), 77-86. Retrieved from http://www.scielo.org.co/pdf/bsaa/v10n1/v10n1a10.pdf
Grosso, G. S. (2017). Origin Nature Nature Physicochemical Properties And therapeutic value of propolis.(April). Retrieved from https://www.researchgate.net/publication/316271304_Origen_naturaleza_propiedades_fisicoquimicas_y_valor_terapeutico_del_propoleo#fullTextFileContent
Julián Martínez, G., Carlos Garcia, P., Diego Durango, R., & Jesús Gil, G. (2012). Characterization of propolis from the municipality of Caldas obtained by two collection methods. Revista MVZ Cordoba, 17(1), 2861-2869. Retrieved from https://revistamvz.unicordoba.edu.co/article/view/254/323
Kozlowski, T. T. (2002). IRAM 15359 Argentine standard for propolis. Forest Ecology and Management, 158(12), 195-221.
-Kumazawa, S., Hamasaka, T., & Nakayama, T. (2004). Antioxidant activity of propolis of various geographic origins. 84, 329-339. https://doi.org/10.1016/S0308-8146(03)00216-4.
Lemus, M., Rodríguez, R. J. de, & Orellana, H. R. de. (1995). Salvadoran standard nso 67.03.01.01:01. 11. Retrieved from https://www.oirsa.org/contenido/2017/El_Salvador_INOCUIDAD/8. NSO 65 19 02 03 - QUALIDAD_DE_PROPOLEO_CRUDO.pdf
Mercedes, A. De, & Modroño, M. (2019). Propolis applications in organic farming and viticulture. 1-8.
Morales, W. F. (2012). Scientific evidence of propolis from the medical point of view. In EVIDENCIA CIENTÍFICA DEL PROPÓLEOS DESDE EL PUNTO DE VISTA MEDICAL. Retrieved from http://www.propoleo.cl/cientificospropolis/walter_fierro.pdf
Muñoz, L., Linares, S. and Narváez, W. (2011). Propolis Properties As Functional Natural Additive on Animal Nutrition. Biosalud, 10(2), 101-111. Retrieved from http://www.scielo.org.co/pdf/biosa/v10n2/v10n2a10.pdf
Núñez R., D., Balboa P., N., Alvear Z., M., Ceron N., A., Abarzua S., K., & Vasconcellos C., A. (2018). Evaluation of the anti-inflammatory activity of Chilean propolis on histological sections of mouse ears. International Journal of Morphology, 36(1), 189-193. https://doi.org/10.4067/S0717-95022018000100189.
Palomino G, L. R., García P, C. M., Gil G, J. H., Rojano, B. A., & Durango R, D. L. (2009). Determination of phenol content and evaluation of antioxidant activity of propolis collected in the department of Antioquia (Colombia). Vitae, 16(3), 388-395. Retrieved from http://www.scielo.org.co/scielo.php?pid=S0121-40042009000300013&script=sci_abstract&tlng=es
Patiño, C. L. (2001). Globalization and production of propolis in Colombia and Latin America. IV International Seminar on Africanized Bee, VIII Beekeepers Meeting, 0, 25-38. Retrieved from http://www.scielo.org.co/pdf/bsaa/v9n1/v9n1a15.pdf
Penadillos, R., & Antonio, R. (2013). Quantification of flavonoids in the ethanol extract of propolis. Revista Pharmaciencia December, 1(2), 51-56.
Rodríguez, L. P., & Nuñez, I. C. (2014). Preliminary characterization of 10% propolis jelly for stomatological use. Retrieved from https://www.researchgate.net/publication/290447534_Revista_cubana_de_Quimica_Vol_XXVI_No_2_mayo-agosto2014
Rodríguez Martín, E. (2014). Author: Vanesa Noriega Salmón. El propoleo,otro recursto terapeutico en la practica clinica. , 27. Retrieved from https://repositorio.unican.es/xmlui/bitstream/handle/10902/5580/NoriegaSalmonV.pdf?sequence=1&isAllowed=y
Salamanca Grosso, G. (2017). Origin, nature, physicochemical properties and therapeutic value of propolis.
Sigurdsson, H., Houghton, B., McNutt, S., Rymer, H. and Stix, J. (2000). Brazilian regulations for animal products DIPOA. Encyclopedia of volcanoes. , 2000, 662.
Tagliacollo, V. A. (2014). Quality of propolis commercialized in the informal market Quality of propolis commercialized in the informal market Qualidade da própolis comercializada no mercado informal. https://doi.org/10.1590/S0101-20612011000300031